Object:
@ES Elevation of serum IgE is the most characteristic immune response in helminthic
infections. Recently, expression of CD23(Fc RII) on B lymphocytes play a major role in IgE
production in allergic diseases. However, the mechanisms causing increased IgE production
during helminthic infections are pooly understood. In the present study, the expression of CD
23 on splenic B lymphocytes during the course of infection with Paragonimus westermani
was examined.
@ES Methods:
@EN Female, 4-6-week old BALB/c mice were inoculated orally with 20 metacercariac of P.
westermani. For detection of CD23 positive B lymphocytes by flow cytometry, splenocytes
from infected mice and non-infected age matched controls were stained with FITC-conjugated
rat anti-mouse CD23 and PE-conjugated rat anti-mouse CD45R/B220 mono-clonal antibody.
And also, to observe the effect of metacercarial ESP on the expression of CD23 antigen,
splenocytes from non-infected mice were incubated in the presence of ESP at 37℃ for 6 h
and 24 h.
@ES Results:
@EN The frequency of CD23 positive B lymphocytes of infected mice was increaed
significantly(P<0.05) at two and three weeks after infection(43.4±7.52% and 44.4±2.99%,
respectively) and persisted the higher levels at four and six weeks after infection. Expression
of CD23 antigen of cultured splenocytes from non-infected mice in the presence of
metacercarial ESP was increased significantly(P<0.05) at 24 h after incubation(60.1±7.54%).
@ES Conclusion:
@EN These results suggest that the expression of CD23 antigen induced by metacercarial
ESP might play a importanat role in serum IgE production in mice infected with P.
westermani.
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